Loading...
|
Please use this identifier to cite or link to this item:
http://140.112.114.62/handle/246246/99423
|
| Title: | Establishment and Characterization of a Human Gastric Carcinoma Cell Line Tmc-1 |
| Authors: | 徐榮源;姜淑媛;鍾尹禎;鄭高珍;林奇宏;俞志誠;吳銘芳;張子明
SHYU, RONG-YAUN;JIANG, SHUN-YUAN;JONG, YIIN-JENG;CHENG, KUO-CHEN;LIN, CHI-HUNG;YU, JYH-CHERNG;WU, MING-FANG;CHANG, TZU-MING |
| Contributors: | 動物中心 |
| Keywords: | gastric cancer;cell line;interferon;karyotypic aberration |
| Date: | 2004 |
| Issue Date: | 2009-01-19T02:41:43Z
|
| Abstract: | Established cancer cell lines are useful in the study of various cancers. We established a human gastric carcinoma cell line TMC-1 derived from the lymph node of a moderately differentiated adenocarcinoma of the stomach. TMC-1 cells grew in vitro as a mixture of attached and suspension cells, and exhibited spindle or ovoid morphology. They had a population doubling time of 15 h, a plating efficiency of 61 %, formed colonies in semisolid agar, secreted the tumor marker CA 19-9, and were tumorigenic in athymic nude mice. The cells expressed E-cadherin and beta-catenin. The karyotypic analysis demonstrated hyperdiploid features with a modal chromosome of 53 . The cell had the deletion at chromosome 18q and gains at chromosome 2p13 -25, 5p15, 5q21- 35, 7, 8q24, 9q, 11, 12p, 14q24-32 and 20. Analysis by fluorescence in situ hybridization showed the deletion at 7 qtel and duplication at 7q11.2 at the rearranged chromosome 7. Growth of TMC-1 cells was inhibited by 27-32% by interferon-alpha (2,000 U/ml) and by interferon-gamma with an IC50 of 125 U/ml. The cell line is tumorigenic in vivo, and its growth is moderately inhibited by interferon-alpha and interferon-gamma. It can be used to develop new modalities of human gastric cancer treatment. Copyright (C) 2004 S. Karger AG, Basel. |
| Relation: | CELLS TISSUES ORGANS v.177 n.1 pp.37-46 |
| Appears in Collections: | [實驗動物中心] 期刊論文
|
Files in This Item:
There are no files associated with this item.
|
All items in NTUR are protected by copyright, with all rights reserved.
|
