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http://ntur.lib.ntu.edu.tw/handle/246246/94572
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| Title: | Hla-Dpb1*0201 Allele Is Negatively Associated with Immunoglobulin E Responsiveness Specific for House Dust Mite Allergens in Taiwan |
| Authors: | 胡忠怡;許秉寧;林榮華;謝貴雄;蔡考圓
HU, CHUNG-YI;HSU, PING-NING;LIN, RONG- HWA;HSIEH, KUE-HSIUNG;TSAI, KAW-YAN |
| Contributors: | 醫學檢驗暨生物技術學系 |
| Keywords: | HLA-class II genes;PCR/SSOPH;house dust mite (HDM);Der p1, Der p2, Der p5;allergen-specific IgE |
| Date: | 2000 |
| Issue Date: | 2009-09-23T04:30:21Z |
| Abstract: | Background: House dust mite (HDM) Dermatophagoides pteronyssinus (Dp) is the most important source of indoor allergens that cause allergic diseases in Taiwan. We prepared purified HDM allergens (Der p1, Der p2 and Der p5) to detect allergen-specific IgE responsiveness among large number of test subjects. The robust genetic typing system for HLA class II genes also facilitated the study on association of HLA and allergic response toward HDM. Objective: This study intended to investigate the association between HLA class II alleles and the IgE responsiveness to the major allergens from HDM, Dp. Method: Two hundred and forty-eight subjects were selected for HLA association study. Plasma HDM allergens (Der p1, Der p2, Der p5)- specific IgE and Der p2-specific IgG antibodies were detected by ELISA, while HLA class II -DRB1, -DQA1, -DQB1, - DPB1 genetic polymorphism was determined by polymerase chain reaction / sequence-specific oligonucleotide probe hybridization (PCR/SSOPH). Statistical comparison of the allelic distribution of each HLA class II genes among the individuals with/without HDM allergens-specific IgE and IgG antibodies were performed . Results: There was no significant association between HLA DRB1, DQB1, DQA1 alleles and HDM- specific IgE responsiveness noted. Only DRB1*0803 and the linked DQA1*0103 alleles showed positive association with Der p5- specific IgE responsiveness. However, we found that HLA-DPB1*1301 predisposed subjects to IgE responsiveness to HDM Der p5. HLA DPB1*0501 was weakly associated with the IgE responsiveness to HDM Der p1 and Der p5 . There was a strong negative association between the HLA-DPB1*0201 allele with IgE responsiveness to Der p1 (OR: 0.30, p<0.0001, p<0.0007, pc<0.010 ). Conclusion: We clearly observed the association between HLA DPB1 alleles and specific IgE responsiveness to HDM major allergens. The molecular mechanism of HLA-DPB1* 0201 involvement in protecting subjects from HDM-specific IgE responsiveness await further investigation. |
| Relation: | CLINICAL AND EXPERIMENTAL ALLERGY v.30 n.4 pp.538-545 |
| Appears in Collections: | [Dept. of Clinical Laboratory Sciences and Medical Biotechnology] Periodical Articles
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