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http://ntur.lib.ntu.edu.tw/handle/246246/94570
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| Title: | Regulatory Roles of Spnt, a Novel Gene Located within Transposon Tntir |
| Authors: | 魏君容;蘇伯琦;洪育芝;謝尚諶;賴信志
WEI, JIUN-RUNG;SOO, PO-CHI;HORNG, YU- TZE;HSIEH, SHANG-CHEN;LAI, HSIN-CHIH |
| Contributors: | 醫學檢驗暨生物技術學系 |
| Keywords: | Serratia marcescens;sliding;quorum-sensing;SpnT;DNA replication |
| Date: | 2006 |
| Issue Date: | 2009-09-23T04:22:29Z |
| Abstract: | The transposon TnTIR contains spnIR quorum-sensing system regulating sliding motility and the production of nuclease, biosurfactant, and prodigiosin in Serratia marcescens. Within TnTIR, a gene named spnT is upstream of and co- transcribed with spnI SpnT is a cytoplasmic protein and its level peaks during early stationary phase. spnT over- expression resulted in inhibition of sliding motility and synthesis of prodigiosin, and biosurfactant similar to spnR. spnT but not spnR over-expression induced cell elongation and aberrant DNA replication in S. marcescens and Escherichia coli strains. In comparison with wild-type E coli strain, over -expression of spnT in an E coli priA and dnaC double-mutant strain did not lead to the aberrant cell morphology phenotypes, suggesting SpnT may act through the recombination-dependent DNA replication system. As spnT over -expression inhibited swarming but not swimming motility, SpnT may indirectly function as a negative regulator of surface-dependent migration and secondary metabolite production. (c) 2006 Elsevier Inc. All rights reserved. |
| Relation: | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS v.348 n.3 pp.1038- 1046 |
| Appears in Collections: | [醫學檢驗暨生物技術學系] 期刊論文
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