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http://ntur.lib.ntu.edu.tw/handle/246246/94566
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| Title: | Fas-Ligand-Expressing Adenovirus-Transfected Dendritic Cells Decrease Allergen-Specific T Cells and Airway Inflammation in a Murine Model of Asthma |
| Authors: | 莊雅惠;孫昭玲;江伯倫
CHUANG, YA-HUI;SUEN, JAU-LING;CHIANG, BOR-LUEN |
| Contributors: | 醫學檢驗暨生物技術學系 |
| Keywords: | OVA-specific T cells;Adenoviral vector;CD95 ligand;Apoptosis;Gene therapy |
| Date: | 2006 |
| Issue Date: | 2009-09-23T04:11:31Z |
| Abstract: | T cells expressing a type-2 T helper profile of cytokines ( Th2 cells) have been demonstrated to play an important role in the initiation and progression of allergic asthma, and it is well known that Fas ligand (FasL ) induces apoptosis when bound to its receptor, Fas. In the present study, we examined the possibility of modulating asthma manifestations by dendritic cells (DCs) genetically engineered to express FasL (DC-FasL), which could deliver a death signal to T cells in an antigen-specific manner. The delivery of DC-FasL into ovalbumin (OVA)-immunized allergic mice decreased the airway hyper-responsiveness(AHR). Moreover, we established a mouse model of airway inflammation by using an adoptive transfer of Th2 cells derived from ovalbumin T cell receptor transgenic mice to study the effect of DC-FasL on airway reactivity. The administration of DC-FasL in Th2-cell- induced allergic mice had significantly decreased AHR, airway inflammation, and IL-4, IL-5 and IL-13 production. Furthermore, the numbers of OVA-specific T cells were decreased in the lung of mice receiving DC-FasL. These results demonstrate that FasL-expressing dendritic cells might be applied for the modulation of allergic responses. |
| Relation: | JOURNAL OF MOLECULAR MEDICINE-JMM v.84 n.7 pp.595-603 |
| Appears in Collections: | [Dept. of Clinical Laboratory Sciences and Medical Biotechnology] Periodical Articles
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