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| Title: | C2gnt-M Is Downregulated in Colorectal Cancer and Its Re-Expression Causes Growth Inhibition of Colon Cancer Cells |
| Authors: | 黃敏銓;黃約翰;梁金銅;沈湯龍;何肇基;許世明
HUANG, MIN-CHUAN;HUANG, JOHN;LIANG, JIN-TUNG;SHEN, TANG-LONG;HO, CHAO-CHI;HSU, SU-MING |
| Contributors: | 解剖學暨細胞生物學科所 |
| Keywords: | C2GnT-M;colon cancer;apoptosis;migration;invasion |
| Date: | 2006 |
| Issue Date: | 2009-09-22T08:09:25Z |
| Abstract: | Changes in carbohydrates on the cell surface are associated with tumor malignancy. The mucin-type core 2 beta-1,6- N- acetylglucosaminyltransferase (C2GnT-M) is highly expressed in the gastrointestinal tract and catalyses the formation of core 2, core 4, and blood group I branches on O-glycans. In the present study, we evaluated the role of C2GnT-M in colorectal cancer. C2GnT-M downexpression was observed in 73 .6% of the primary tumors from colorectal cancer patients ( 39 of 53) analysed by cancer pro. ling array. Consistently, the majority of colon cancer cell lines and primary colon tumors expressed lower levels of C2GnT-M than did normal colon tissues by RT-PCR. HCT116 cells stably transfected with C2GnT-M inhibited expression of the core 1 structure, Gal beta 1,3GalNAc alpha 1-Ser/Thr, on the cell surface. Moreover, C2GnT-M expression suppressed cell adhesion, motility, and invasion as well as colony formation ability. The growth of C2GnT-M-transfected HCT116 and SW 480 cells was dramatically suppressed, and the cell death induced by C2 GnT-M was demonstrated by an increase in the annexin V- positive cells. Interestingly, C2GnT-M inhibited cell adhesion to collagen IV and fibronectin, and decreased tyrosine phosphorylation of paxillin, indicating that the changes in cancer behavior may be partly mediated by integrin-signaling pathways. Tumor growth in vivo was also significantly suppressed by C2GnT-M in the xenografts of nude mice. These results demonstrate that C2GnT-M is frequently downregulated in colorectal cancer and suppresses colon cancer cell growth. |
| Relation: | ONCOGENE v.25 n.23 pp.3267-3276 |
| Appears in Collections: | [Dept. of Anatomy and Cell Biology] Periodical Articles
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