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http://ntur.lib.ntu.edu.tw/handle/246246/94546
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| Title: | Redistribution of Gfap and Ab-Crystallin after Thermal Stress in C6 Glioma Cell Line |
| Authors: | 曾唯嘉;盧國賢;錢宗良
TSENG, WEI-CHIA;LU, KUO-SHYAN;CHIEN, CHUNG-LIANG |
| Contributors: | 解剖學暨細胞生物學科所 |
| Keywords: | aB-crystallin;C6 cell;GFAP;glia;heat shock |
| Date: | 2006 |
| Issue Date: | 2009-09-22T08:07:33Z |
| Abstract: | Some intermediate filament (IF) proteins expressed in the development of glia include nestin, vimentin, and glial fibrillary acidic protein (GFAP). However, GFAP is the major intermediate filament protein of mature astrocytes. To determine the organization of GFAP in glial cells, rat GFAP cDNA tagged with enhanced green fluorescent protein (EGFP) was transfected into the rat C6 glioma cell line. After selection, two stable C6-EGFP-GFAP cell lines were established. Stable C6-EGFP-GFAP cell lines with or without heat shock treatment were analyzed by immunocytochemistry, electron microscopy, and Western blot analysis. In the transient transfection study, EGFP-GFAP transiently expressed in C6 cells formed punctate aggregations in the cytoplasm right after transfection, but gradually a filamentous structure of EGFP-GFAP was observed. The protein level of nestin in the C6-EGFP-GFAP stable clone was similar to that in the pEGFPC1 transfected C6 stable clones and non-transfected C6 cells, whereas the level of vimentin was reduced in Western blotting. Interestingly, the expression level of small heat shock protein aB- crystallin in C6-EGFPGFAP cells was also enhanced after transfection. Immunostaining patterns of C6-EGFP-GFAP cells showed that GFAP was dispersed as a fine filamentous structure. However, after heat shock treatment, GFAP formed IF bundles in C6- EGFP-GFAP cells. In the meantime, aB-crystallin also colocalized with IF bundles of GFAP in C6-EGFP-GFAP cells. The heat-induced GFAP reorganization we found suggested that small heat shock protein aB-crystallin may play a functional role regulating the cytoarchitecture of GFAP. |
| Relation: | JOURNAL OF BIOMEDICAL SCIENCE v.13 n.5 pp.681-694 |
| Appears in Collections: | [解剖學暨細胞生物學科暨研究所] 期刊論文
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