数据加载中.....
|
jsp.display-item.identifier=請使用永久網址來引用或連結此文件:
http://140.112.114.62/handle/246246/94427
|
| 题名: | Change of Potassium Current Density in Rabbit Corneal Epithelial Cells during Maturation and Cellular Senescence |
| 作者: | 張淑雯;張國志;蘇銘嘉
CHANG, SHU-WEN;CHANG, GWO-JYH;SU, MING -JAI |
| 贡献者: | 藥理學科所 |
| 关键词: | Animals;Cell Aging/ physiology;Epithelial Cells/ metabolism;Epithelium, Corneal/cytology/ metabolism;Potassium Channels, Voltage-Gated/ metabolism;Rabbis |
| 日期: | 2006 |
| 上传时间: | 2009-09-23T08:34:38Z
|
| 摘要: | BACKGROUND: Voltage-gated potassium (K+) channels may participate in cellular developmental regulation, including cell differentiation, proliferation and apoptosis. This study investigated the change of K + current densities in corneal epithelial cells during maturation and cellular senescence. METHODS: New Zealand white rabbits were divided into three age groups: newborn (<or= 7 days old, n = 18); young (8-12 weeks old , n = 59); and adult (20-28 weeks old, n = 16). Rabbit corneal epithelial cells were subdivided into the following three groups: small cells with capacitance < 6.0 pF; medium cells with capacitance 6.0-10.0 pF; and large cells with capacitance > 10.0 pF. Using a whole-cell clamp technique, K+ current was recorded and current densities were calculated. Differences in K+ current densities among newborn, young and adult rabbits, as well as differences among small, medium and large cells, were analyzed. RESULTS: We delineated two types of cells manifesting different amplitudes of depolarization-activated K+ outward currents. The averaged current density of type 1 response cells was significantly larger than that of type 2 cells in newborn, young, and adult groups. For newborn epithelial cells, the depolarization-gated outward K+ current density decreased from small to medium to large cells (p = 0.049, at a membrane potential of 140 mV). A similar pattern of change in current density was also delineated for these cell sizes in young and adult rabbit corneal cells (p < 0.001 for both young and adult rabbits). An increase in depolarization-gated outward K+ current density was also delineated from newborn to young to adult rabbits (p < 0.001, p < 0.001 and p < 0.006 for small, medium and large cells, respectively, at a membrane potential of 140 mV). CONCLUSIONS: Corneal epithelial cells expressed K+ channel densities that were distinct from basal to superficial cells and from newborn to adult rabbits. |
| 關聯: | JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION v.105 n.1 pp.7-16 |
| 显示于类别: | [藥理學科暨研究所] 期刊論文
|
在NTUR中所有的数据项都受到原著作权保护.
|
