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Please use this identifier to cite or link to this item:
http://140.112.114.62/handle/246246/64132
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| Title: | Establishment and characterization of a tumorigenic cell line from areca quidand tobacco smoke-associated buccal carcinoma. |
| Authors: | 郭彥彬 |
| Contributors: | 臨床牙醫學研究所 |
| Keywords: | oral cancer |
| Date: | 2007-08 |
| Issue Date: | 2008-02-21T03:10:55Z
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| Abstract: | A cell line, TW2.6, has been established from the surgically resected specimen of an untreated primary squamous cell carcinoma of the buccal mucosa from a 48-year-old man who was an areca quid chewer and tobacco smoker. TW2.6 cells exhibited morphological features of keratinocytes and replicated rapidly in culture with a doubling time of 24h. The karyotype showed human chromosomes with high hyperdiploidy and complex rearrangements. Western blotting showed pronounced expression of p53 and moderate expression of p21(CIP1). The baseline expressions of p27(KIP1) and p16(INK4a) were barely detectable. Low levels of Bax and Fas were found in TW2.6 cells but Bcl-2 expression was more readily observed. Mutational analysis of p53 gene revealed an A-->G transition at the second base of codon 220, resulting in amino acid substitution from tyrosine to cysteine in the protein. Functional analysis showed that TW2.6 was unable to activate the p53-specific PUMA promoter. Lipofectamine 2000 and calcium phosphate precipitation technique offer good transfection efficiencies for TW2.6 cells and may be used in future transfection experiments. A xenograft-SCID mouse tumor model was established for TW2.6. Histological examination demonstrated that the engrafted tumors maintained the morphological features of a squamous cell carcinoma. It is thought that the establishment of tumorigenic TW2.6 cell line provides a valuable model for AQ and tobacco smoke-associated buccal carcinoma. |
| Relation: | Oral Oncology |
| Appears in Collections: | [臨床牙醫學研究所] 期刊論文
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