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http://ntur.lib.ntu.edu.tw/handle/246246/22581
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| Title: | 電磁場刺激對造骨細胞影響之機制探討 |
| Authors: | 孫瑞昇 |
| Keywords: | Osteoblasts;PEMF;collagen;osteocalcin, RANKL |
| Date: | 2004 |
| Publisher: | 臺北市：國立臺灣大學醫學院(附設醫院)醫學工程部 |
| Abstract: | The use of electricity in the medicine was
accelerated in 1953 when Yasuda induced osteogenesis by electrical stimulation. Increased osteogenesis has been reported using all major forms of electrical stimulation; direct current, capacitively coupled and inductively coupled electromagnetic fields. The
manner in which electric fields influence
the behavior of cells remains poorly understood. The purposes of this research were to assess the stimulatory effect of pulsed electromagnetic field (PEMF) on bone cells and to validate
that bone tissue-like formation was associated with the increase in the number of cells and/or with the enhancement of cellular differentiation.
The osteoblast-like cells were isolated and pulsed electromagnetic field (PEMF) stimulation was stimulated from the 3rd to 14th days with a duration of 8 hrs/ day.
The proliferation of osteoblasts was assessed by colorimetric MTT assay, and the synthesis and secretion of alkaline phosphatase (ALP), lactate
dehydrogenase (LDH) by osteoblasts were measured. The differentiation was analyzed by ALP staining and von-Kossa staining; while the gene expression during PEMF stimulation was also
analyzed. The cellular density in the PEMF group increased faster than in the control group until the cells became confluent and started to form multilayers; thereafter, the optic absorption was
indiscernible in both groups.
The ALP concentrations in the PEMF stimulated group decreased over the control group at 3, 5 and 7 days of culture, respectively. Similar result was observed in the von-Kossa staining. In
the presence of on PEMF stimulation, type I collagen, osteocalcin and osteopontin mRNA expression were quite similar to that of control group at the 14 days’ experimental period, while
the OPG mRNA expression was up-regulated and the RANKL mRNA expression were down-regulated than
that of the control. Our result demonstrated that PEMF treatment on osteoblasts may accelerate cellular proliferation, but did not affect cellular differentiation, maturation and
mineralization nodules formation. The PEMF stimulated increase in the bone tissue-like formation was most likely associated with the increase in the number of cells, but not with the
enhancement of the osteoblasts differentiation. |
| Appears in Collections: | [附設醫院醫學工程部] Research Project
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