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http://ntur.lib.ntu.edu.tw/handle/246246/170418
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| Title: | Interleukin-6 Promotes Cervical Tumor Growth by Vegf-Dependent Angiogenesis Via a Stat3 Pathway |
| Authors: | 魏凌鴻;郭明良;陳祈安;李建南;謝長堯
WEI, LIN-HUNG;KUO, MIN-LIANG;CHEN, CHI-AN;LEE, CHIEN-NAN;HSIEH, CHANG-YAO |
| Contributors: | 腫瘤醫學部 |
| Date: | 2003 |
| Issue Date: | 2009-10-13T15:13:01Z |
| Abstract: | Interleukin-6 (IL-6) has received particular attention in the pathogenesis of cervical cancer, although the underlying mechanism remains elusive. This study revealed that IL-6 promotes in vivo tumor growth of human cervical cancer C33A cells, but does not substantially alter their in vitro growth kinetics. The in vivo angiogenic assays showed that IL-6 increases angiogenic activity in human cervical cancer cells, an effect that is specifically associated with upregulation of vascular endothelial growth factor (VEGF). Also, using anti-VEGF antibody to block VEGF function significantly inhibited IL-6-mediated angiogenesis and tumor growth in nude mice, strongly supporting the critical role of VEGF in the IL-6-mediated cervical tumorigenesis. Accordingly, the signaling pathway downstream of IL-6/IL-6R responsible for the regulation of VEGF was investigated. Notably, pharmacological inhibition of PI3-K or MAPK failed to inhibit IL-6-mediated transcriptional upregulation of VEGF. Meanwhile, blocking STAT3 pathway with dominant- negative mutant STAT3D effectively abolished IL-6-induced VEGF mRNA. In transient transfections, a luciferase reporter construct containing the full-length 1.5-kb VEGF promoter or a 1 .2-kb fragment lacking the known hypoxic-response element also exhibited the same degree of response to IL-6. Additionally, transient transfection of STAT3D downregulated the 1.2-kb VEGF promoter luciferase reporter stimulated by IL-6. Based on the above phenomenon combined with the concomitant increased tumor expression of IL-6 and VEGF in cervical cancer tissues, we conclude that IL-6 may promote cervical tumorigenesis by activating VEGF-mediated angiogenesis via a STAT3 pathway. |
| Relation: | ONCOGENE v.22 n.10 pp.1517-1527 |
| Appears in Collections: | [附設醫院腫瘤醫學部] 期刊論文
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