 |
English
|
正體中文
|
简体中文
|
全文笔数/总笔数 : 43236/136804
造访人次 : 2068630
在线人数 : 61
|
|
|
数据加载中.....
|
jsp.display-item.identifier=請使用永久網址來引用或連結此文件:
http://ntur.lib.ntu.edu.tw/handle/246246/163759
|
| 题名: | A reduced oxygen tension (5%) is not beneficial for maintaining human embryonic stem cells in the undifferentiated state with short splitting intervals |
| 作者: | Chen, Hsin-Fu;Kuo, Hung-Chih;Chen, Wann-hsin;Wu, Fang-Chun;Yang, Yu-Shih;Ho, Hong-Nerng
陳信孚;郭紘志;陳婉昕;楊友仕;何弘能 |
| 贡献者: | 臨床醫學研究所 |
| 日期: | 2009-01 |
| 上传时间: | 2009-08-14T06:45:38Z |
| 摘要: | Human embryos grow naturally in vivo in lower oxygen (O-2) tension environments than atmospheric O-2 tension. Therefore , human embryonic stem cells (hESC), a derivative of embryos , will likely grow more favorably in a reduced O-2 tension. This study aimed to compare the behavior of hESC under reduced O-2 tension (5%) versus normoxia (21%). hESC lines were cultured in different O-2 tensions and then examined for morphology, apoptosis and gene expression profiles. hESC grown in 5% O-2 tension were not morphologically different from hESC grown in normoxia on day 7 of the first and fourth passages. However, after prolonged culture without splitting (10-14 days), hESC colonies were thinner and looked better morphologically in 5 % O-2, but the cells proliferated more slowly and their sizes were larger. At most time points, the gene expression profiles in both O-2 tensions showed no major difference in representative stemness genes (Oct-3/4, Nanog and Cripto), differentiation genes (Desmin, Nestin, alpha-fetoprotein and GDF-9) and hypoxia-related genes (HIF-1 alpha and VEGF). A lower level of cyclin-D1 mRNA (suggestive of less Wnt pathway signaling on day 7 of the fourth passage) and a higher level of Desmin (suggestive of more differentiation to mesoderm, at day 7 of the first passage) were detected in 5% O-2. This study suggests that for routine culture of hESC with a short splitting interval (7 days), a low O-2 tension (5% O-2) probably does not provide significant advantages over the standard 21% O-2 tension for the maintenance of an undifferentiated state by the criteria used in this study. |
| 關聯: | HUMAN REPRODUCTION v.24 n.1 pp.71-80 |
| 显示于类别: | [臨床醫學研究所] 期刊論文
|
在NTUR中所有的数据项都受到原著作权保护.
|