DSpace community: 附設醫院影像醫學部

Morphological and Topological Characteristics of Coronary Venous System in Chronic Systolic Heart Failure

title: Morphological and Topological Characteristics of Coronary Venous System in Chronic Systolic Heart Failure

Cervical Abscess with Vaginal Fistula after Extraperitoneal Cesarean Section

title: Cervical Abscess with Vaginal Fistula after Extraperitoneal Cesarean Section abstract: Extraperitoneal cesarean section was once used for the prevention of infection and postoperative adhesion. However, we report an unusual complication after this procedure. A 29-year-old woman had pus discharge from the anterior vaginal wall after extraperitoneal cesarean section. Broad- spectrum antibiotics failed to relieve her symptoms and vaginal culture yielded Morganella morganii. Magnetic resonance imaging, sagittal view, showed a cervical abscess measuring 5 x 5 cm with a tract extending to the anterior vagina. After performing dilation and abscess drainage via the cervical ostium, the symptoms gradually subsided with adequate antibiotic treatment. Cervical abscess may develop after extraperitoneal cesarean section and present initially as vaginal fistula. Detailed imaging study provides comprehensive anatomic information for effective management.

Segmental Anatomy of Coronary Arteries

title: Segmental Anatomy of Coronary Arteries

Magnetic Nanoparticle Labeling of Mesenchymal Stem Cells without Transfection Agent: Cellular Behavior and Capability of Detection with Clinical 1.5 T Magnetic Resonance at the Single Cell Level

title: Magnetic Nanoparticle Labeling of Mesenchymal Stem Cells without Transfection Agent: Cellular Behavior and Capability of Detection with Clinical 1.5 T Magnetic Resonance at the Single Cell Level abstract: The purpose of this work was to evaluate the efficacy of labeling human mesenchymal stem cells (hMSCs) by ionic superparamagnetic iron oxide (SPIO ) without a transfection agent and verifying its capability to be detected with clinical 1.5 T magnetic resonance (MR) at the single-cell level. Human hMSCs were incubated for 24 h with an ionic SPIO, Ferucarbotran. The labeling efficiency of hMSCs was determined by iron content measurement spectrophotometrically, and the influence of labeling on cell behavior was ascertained by examination of cell viability using the trypan blue exclusion method, cell proliferation analysis using MTT (3-(4,5- Dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide) assay, mitochondrial membrane potential (MMP) change, differentiation capacity, and reactive oxygen species (ROS) production measured by dichlorofluorescein diacetate (DCFDA) fluorescent probe. Labeled hMSCs were scanned under 1.5 T MRI with three- dimensional (3D) and two- dimensional (213) T-2-weighted gradient echo (GRE) pulse sequences. Human hMSC labeling without transfection agent was efficient. The iron content in hMSCs was 23.4 pg Fe/cell. No significant change was found in viability , proliferation, MMP change, ROS production, or differentiation capacity. About 45.2% of the hMSCs could be detected using 1.5 T MRI at the single cell level with 3D GRE and four repetitions.