DSpace community: 實驗動物中心

Establishment and Characterization of a Human Gastric Carcinoma Cell Line Tmc-1

title: Establishment and Characterization of a Human Gastric Carcinoma Cell Line Tmc-1 abstract: Established cancer cell lines are useful in the study of various cancers. We established a human gastric carcinoma cell line TMC-1 derived from the lymph node of a moderately differentiated adenocarcinoma of the stomach. TMC-1 cells grew in vitro as a mixture of attached and suspension cells, and exhibited spindle or ovoid morphology. They had a population doubling time of 15 h, a plating efficiency of 61 %, formed colonies in semisolid agar, secreted the tumor marker CA 19-9, and were tumorigenic in athymic nude mice. The cells expressed E-cadherin and beta-catenin. The karyotypic analysis demonstrated hyperdiploid features with a modal chromosome of 53 . The cell had the deletion at chromosome 18q and gains at chromosome 2p13 -25, 5p15, 5q21- 35, 7, 8q24, 9q, 11, 12p, 14q24-32 and 20. Analysis by fluorescence in situ hybridization showed the deletion at 7 qtel and duplication at 7q11.2 at the rearranged chromosome 7. Growth of TMC-1 cells was inhibited by 27-32% by interferon-alpha (2,000 U/ml) and by interferon-gamma with an IC50 of 125 U/ml. The cell line is tumorigenic in vivo, and its growth is moderately inhibited by interferon-alpha and interferon-gamma. It can be used to develop new modalities of human gastric cancer treatment. Copyright (C) 2004 S. Karger AG, Basel.

Triflavin, an Arg-Gly-Asp-Containing Peptide, Inhibits Human Cervical Carcinoma (HeLa) Cell-Substratum Adhesion Through an RGD-Dependent Mechanism

title: Triflavin, an Arg-Gly-Asp-Containing Peptide, Inhibits Human Cervical Carcinoma (HeLa) Cell-Substratum Adhesion Through an RGD-Dependent Mechanism abstract: 　　Triflavin, an Arg-Gly-Asp-cintaining peptide, inhibits human cervical carcinoma (HeLa) cell-substratum adhesion through an RGD-dependent mechanism. PEPTIDES 15(8) 1391-1398 , 1994.-Triflavin, 1 7.5-kDa cysteine- rich polypeptide purified from Trimeresurus flavoviridis snake venom, belongs to a family of RGD-containing peptides, termed disintegrins , that have been isolated from the venoms of various vipers and shown to be potent inhibitors of platelet aggregation. The interaction of tumor cells with extracellular matrices such as fibronectin, vitronectin, and collagen has been shown to be mediated through a family of cell surface receptors that specifically recognize an arginie-glycine- aspartic acid (RGD) sequence within each adhesive protein. In this study, we show that triflavin dose-dependently inhibited adhesion of human cerical carcinoma ( HeLa) cells to extracellular matrices (ECMs; i.e., fibronectin, fibrinogen , and vitronectin). On the other hand, triflavin exerted a limited inhibitory effect on cell adhesion to laminin and collagen (type I and IV) . On a molar basis, triflavin is approximately 800 times more potent than Gly- Arg-Gly-Asp-Ser (GRGDS) at inhibiting cell adhesion. When immobilized on plate, triflavin significantly promoted HeLa cell adhesion, and this attachment was inhibited by GRGDS. Furthermore, FITC-conjugated triflavin bound to cells in a saturable manner and its binding was inhibited by GRGDS. In addition, triflavin did not affect [3H]thymidine uptade of HeLa cells during 1 3-day incubation. These results suggest that triflavin probably binds to integrin receptors expressed on HeLa cell surface via its RGD sequence within its molecule, thereby inhibiting the adhesion of extracellular matrices to HeLa cells.#0844#

Phrenic Nerve Transfer in the Repair of Brachial Plexus Injuries:An Animal Model

title: Phrenic Nerve Transfer in the Repair of Brachial Plexus Injuries:An Animal Model abstract: Ten young mongrel dogs underwent unilateral denervation of the brachial plexus. In six dogs,a 2-cm segment of phrenic nerve autograft was sutured to either the resected musculocutaneous nerve or the radial nerve. A hemoclip was applied to either musculocutaneous or radial nerve in the contral groups. Five months postoperatively,the grafted musculocutaneous nerve demonstrated less fibrous tissue and less muscle atrophy of the biceps when compared to the control group with clipped nerve. In the group with the grafted radial nerve,the electromyographic findings of multiphasic action potential and muscle contraction from electric stimulation suggested reinnervation of the radial nerve. In conclusion: phrenic nerve transfer may be used to repair specific damages to nerve trunk with histological , electromyographic and clinical recovery.

Triflavin, an antiplatelet peptide, inhibits tumor cell-extracellular matrix adhesion through an arginine-glycine-aspartic acid-dependent mechanism

title: Triflavin, an antiplatelet peptide, inhibits tumor cell-extracellular matrix adhesion through an arginine-glycine-aspartic acid-dependent mechanism abstract: 　　The interaction of fumor cells with extracellular matrix components such as laminin, fibronectin, and collagen has been shown to be mediated through a family of cellsurface receptors that specifically recognize an arginine-glycine- asparfic acid amino acid sequence within each protein. Triflavin, a 7.5 kDa cysteine-rich polypeptide purified from Trimeresurus flavoviridis snake venom, belong to a family of arginine-glycine-aspartic acid-containing peptides termed disintegrins that have been isolated from the venoms of various vipers and shown to be potent inhibitors of platelet aggregation. In this study, we showed that triflavin inhibited adhesion of human hepatoma J-5 cells to extracellular matrices (fibronectin, vitronectin, fibrinogen , and collagen type 1) in a dose-dependent manner. On the other hand, trifiavin exeried a limited inhibitory effect on cell attachment to collagen type IV and laminin ( 40%). Triflavin is approximately 1000 times more potent than glycine-arginine-glycine- aspartic acid-serine at inhibiting cell adhesion, When immobillzed on plate, triflavin promoted J-5 cell attachment ; this attachment was inhibited by glycine-arginine-glycine-aspartic acid-serine. In addition, triflavin labeled with iodine 125 binds to j-5 cells in a saturable manner and its binding was also inhibited by glycine-arginine-glycine-aspartic acid-serine. Its Kd value was estimated to be 3.9×10-7 mol/L amd tje mi, ber pf bomdomg sotes qas arpimd 60,000 per cell. Furthermore, triflavin did not affect tritiated thymidine uptake during a 3-day incubation. These results suggest that triflavin binds by means of its arginine-glycine- aspartic acid amino acid sequence to integrin receptor expressed on the J- 5 cell surface, inhibiting the adhesion of extracellular matrices to tumor cells.#0842#